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The sample is treated with sodium chlorite to isolate holocellulose, rinsed, then treated with sodium hydroxide, and rinsed again (solvent extraction may be required depending on sample preservation).

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Marine shell samples are routinely etched with dilute HCl to minimise the possibility of contamination through isotopic exchange between the sample and its environment.

All aragonitic shell samples are tested for recrystalisation prior to dating to ensure no chemical alteration has taken place.

More information on reservoir effects can be found here.

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